The fluorescence intensity was detected by flow cytometry,and the leakage of CFSE and its dyeing were observed by fluorescence antibody labeling.

• 采用流式细胞术追踪检测标记的IPE细胞的荧光强度变化。 用免疫荧光抗体标记法检测此染色剂有无渗漏以及是否对周围细胞着染。