The focal cerebral ischemia model was produced by occluding middle cerebral artery(MCA) for 2h and reperfusing 24h. Cell apoptosis was detected by Tunel,the expression of bcl-2 and bax were analyzed by immunohistochemical.

• 线栓法制备大鼠大脑中动脉阻塞模型，缺血2h再灌注24h后观察PAMD对脑缺血后细胞凋亡及相关基因bcl-2和bax表达的影响，Tunel法测细胞凋亡，免疫组化法观察bcl-2和bax的表达。