The fragment containing ubi-53 gene was inserted into pET-28a expression vector, expression of the fusion protein was induced by IPTG in E. Coli BL21 (DE3) . The fusion protein was identified by Western blot with a mouse Ab against bovine Ubiquitin.

• 将甜菜夜蛾的ubi-53基因克隆到原核表达载体pET-28a上，转化至大肠杆菌BL21（DE3）中，用IPTG进行诱导表达，用异源泛素单克隆抗体进行Western blot检测，证明了原核表达蛋白是目的蛋白。