The fragment was introduced to pBI121 generating pBI35S-AMF which contained CaMV35S promoter, and then pBI35S-AMF was transferred into Agrobacterium tumefaciens strain LBA4404 and EHA105 through the assistant plasmid pRK2013/HB101 by triparental cross.
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- 把该反义片段连接至pBI121双元载体质粒上得到CaMV35S组成型启动子的表达载体pBI35S-AMF;随后利用"三亲杂交"法将pBI35S-AMF质粒转入农杆菌LBA4404和EHA105两个菌株中.