The gender specificity of the constructed male and female archives were high as determined by analysis using Southern blotting with Digoxigenin(DIG)- labeled male and female cDNA probes.

• 将分别标记荧光素Cy3和Cy的雌虫和雄虫cDNA探针，与制备好的cDNA芯片杂交，用芯片扫描仪扫描，获取每个点的杂交信号，原始信号值经均一化处理。