The images of spores under microscope were captured by area-scan CCD camera,and the gray level images were then transferred into binary images by adjusting threshold.

• 利用面阵CCD摄像机采集显微镜中孢子的图像,通过灰度直方图确定初始阈值,再用二次定值法确定最终阈值,将灰度图像转换成二值图像。