The levels of Prx and TrxR mRNA were determined 24 h and 48 h post,transfection by relative quantitative RT,PCR,and the growth of Trichomonas vaginalis was estimated under microscope 36 h post,transfection.

• 收集转染前、转染后24 h和48 h的毛滴虫细胞,用半定量反转录-PCR(RT-PCR)检测Prx和TrxR的mRNA水平;