The low PCR success rate and poor PCR repeatability of microsatellite alleles due to allelic dropout and false alleles make it difficult to obtain a reliable microsatellite data set.

• 微随体对偶基因的低PCR成功率及低可重复性（即高对偶基因遗失率及高假性对偶基因出现率），使得可靠的微随体资料库难以建立。