The main obejective is to construct an efficiently expressive prokaryotic clone of the second immunodominant epitopes in HTV-1 gp41, detect its products and evaluate it in testing the anti-HTV-1 antibody.
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- 本课题构建了HIV-1 SF2株跨膜蛋白gp41第二优势表位簇基因的原核高效表达克隆,初步检验该融合蛋白抗原性,探讨将其作为侯选多肽用于制备诊断试剂、检测HIV-1抗体的可能性; 并探讨提高原核表达的途径。