The methods of isolating total RNA from plant tissues, including Improved CTAB, SDS-phenol, Hot borate, Guanidine Isothiocyanate and Trizol kit method, were compared and improved.

• 改良CTAB法和改进SDS-酚法可从田间叶片中得到高质量的RNA,改良CTAB法提取的RNA纯度高于SDS-酚法,但产率却显著低于SDS-酚法;