The multiple-attack mechanism of chitin deacetylase (CDA), the character of enzymatic production ofchitosan, and the cloning and expression of CDA gene are reviewed.

• 综述了甲壳素脱乙酰酶(CDA)催化甲壳素脱乙酰基的多点进攻作用机理、酶法制备壳聚糖的特性以及克隆表达CDA基因的研究进展。