The optimal media for subculture callus and differentiation adventitious buds were 1/2MS+2, 4-D lmg/L+6-BA 0.5mg/L+KT 0.5mg/L+ sucrose 30g/L and DCR+6-BA 1.5mg/L+KT 1.5mg/L+ sucrose 20g/L, respectively.

• 1/2MS+2，4-D 1mg/L+6-BA0.；5mg/L+KT 0