The optimum culture conditions of genetically engineered Pichia pastoris in shake flask cultivation and in fed batch fermentation were investigated respectively.

• 在摇瓶条件下对基因工程菌 Pichia pastoris的发酵条件进行了实验 ,并根据摇瓶发酵的优化结果进行了补料分批高密度发酵。