The optimum culture medium of establish regeneration: Callus induced medium is MS add 2,4-D0.5mg/L add BA0.2mg/L. Differentiation medium is MS add BA2.0mg/L add 2,4-D1.0mg/L add KT 0.2mg/L.

• 分化培养基为MS附加BA2.；0mg/L、2；4-D0