The paper review the current situation of mass spectrometry,and introduce ESI and MADLI,which are the key techniques in analysis of protein or macromolecules,GC/MS and LC/MS.

• 本文为质谱技术的研究现状作一综述,着重介绍了ESI、MADLI两种关键的电离技术以及色谱/质谱联用技术,并对未来予以展望。