The plasmid was made? by P. Ghosh, who solved the structure of colicin Ia then years ago (published in Nature). A promoter and SD sequence was introduced into the plasmid.
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- 回答:如果克隆序列有翻译开始信号,是可以用来表达的,但是从该论文公布的克隆序列看,没有由方舟子于2006-1-12,02:18:51: