The positive cell clones had been obtained after selected by G418. The stable transfection and expression of AIRE in HeLa cells were determined by the fluorescence microscope, FACS and Western blotting.

• 继续培养 4周 ,用荧光显微镜观察、流式细胞仪 ( FACS)检测和免疫印迹法检测 AIRE基因在 He La细胞中的稳定表达情况。