The protease from ?Serratia marcescens ?41003(2)was completely inactivated by EDTA at pH7?0 and was reactivated by Ca??2+?,thus,Ca??2+? is essential for the activity of the enzyme.
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- 终浓度为5mmol/L的EDTA可彻底抑制粘质赛氏菌41003(2)胞外蛋白酶活力,而Ca2+可以回复部分酶活力,表明Ca2+为酶活力所必需;