The rMTG was refolded by gel filtration chromatography, The specific activity and the protein concentration of MTG were 21.3U/mg and 75mg/L.

• 用凝胶过滤色谱法对rMTG进行了体外复性，最终得到了复性蛋白浓度为75mg/L和比酶活为21.；3U/mg蛋白的结果。