The recombinant plasmid was transformed into E. coli BL21(DE3), and E. coli BL21(DE3)/pET-LG3 was induced by IPTG.

• 用IPTG诱导,LG3蛋白在大肠杆菌BL21(DE3)中得到了表达,并经Ni-NTA层析柱获得纯化。