The running buffer was 50 mmol/L borax buffer (pH 9.5), the running voltage was 20 kV, and the wave length was 214 nm.

• 最佳电泳条件为:50mmol/L的硼砂(pH 9.；5)缓冲液；20 kV分离电压；214nm紫外检测；环丙沙星为内标。