The set of optimized family-specific primers can amplify the human genes of the Fd and light chains with a high efficiency. These will be helpful for the construction of human Fab antibody libraries.

• 通过本部分工作，在人抗体可变区胚系基因家族分类基础上，根据其5’端保守序列重新分组，优化设计特异性引物，可以保证扩增效率，有利于大容量人源噬菌体Fab抗体库的构建和筛选。