The specific expression of mouse pAdeasy-VIP was verified by RT-PCR and immunofluorescent method in 293 cells after infection with pAdeasy-VIP,but not Ad-EGFP,a similarly constructed control virus.
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- RT-PCR,免疫荧光检测pAdeasy-VIP病毒感染的293细胞,均有VIP的表达。