The specific primers were designed to amplify the coat protein genes of lily symptomless virus(LSV,genus Carlavirus),lily mottle virus(LMoV,genus Potyvirus) and lily virus X(LVX,genus Potexvirus) respectively.

• 设计3对表达引物分别扩增侵染了百合无症病毒(lily symptomless virus,LSV),百合斑驳病毒(lily mottle virus,LMoV)和百合X病毒(lily virus X,LVX)的外壳蛋白基因,并在大肠杆菌BL21(DE3)plysS中原核表达。