The sterols, triterpene alcohols and 4-methylsterols from the unsaponifiables in safflower seed oil were separated and analysed by HPLC, GLC and GS-MS.

• 采用高效液相色谱、气相色谱及气-质联用方法分离和测试红花籽油不皂化物成分，结果表明，红花籽油不皂化物主要成分为甾醇、三萜醇和4-甲基甾醇等。