The stress-inducible transcription factor DREB1A gene is amplified from Arabidopsis thaliana (Columbia ecotype) genome by polymerase chain reaction and cloned into pUC19 vector.
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- 以拟南芥基因组DNA为模板,通过PCR扩增得到逆境胁迫诱导转录因子DREB1A基因,将其克隆到pUC19质粒中。