The supernatant of 20% brain tissue suspension of suckling mice infected with BA66019 strain of XHF virus, were separated by electrophoresis of SDS-PAGE and bloted in NC membranes by western blot technique .

• 用新疆出血热病毒原型毒株BA66019感染乳鼠脑的20%25悬液,经差速离心后的上清为抗原进行SDS-PAGE电泳,再用Westernbolt法将凝胶分离的结果印迹到NC膜上。 将6株单克隆抗体小鼠腹水经饱和硫酸铵纯化后,在NC膜上进行结合反应。