The synthesis for a series of 2,3,7 trihydroxy 9 [(substituent)azo]phenylfluorone and their influence factor of spectrophotometric properties were discussed respectively.

• 对新型2,3,7三羟基9[(取代基)偶氮]苯基荧光酮类试剂的合成及影响该类试剂光度性质的因素进行了讨论,并对其在光度分析中的应用进行了评论。