The target gene was then inserted into expression vector pET-28a and expressed in E.coli BL21 with IPTG induction.

• 将mdlA基因克隆到原核表达载体pET-28a中，转化大肠杆菌BL21(DE3)，经诱导后，重组的脂肪酶在宿主菌中的表达量可达菌体总蛋白量的45.；2%25。