The technique using the automated islet isolator and COBE 2991 for isolation and purification was well-used abroad, but it requires special equipments, and large amounts of expensive collagenase.

• 而目前国外普遍使用的自动分离纯化技术，因需特殊的设备，胶原酶用量多等缺点，在国内尚未普遍使用。