The tender leaves and stems of Eucommia Ulmoides Olive. are incubated in the culture medium of MS+2, 4-D2.0mg/ml so that the cell system of Eucommia Ulmoides Olive. callus is set up.

• 本课题就是在杜仲资源出现紧缺趋势这样的背景下，用组织比较嫩的杜仲叶片和茎尖在MS+2，4-D2.；0mg/ml（2，4-二氯苯氧乙酸）建立了杜仲愈伤组织细胞系；