The typical method of ES cells differentiation into neural cells in vitro includes embryoid bodies (EBs) formation and subsequent differentiation into neural cells under all-trans retinoic acid or serum-free conditions.

• ESCs体外诱导为神经细胞的经典途径是先通过拟胚体阶段以启动分化，再通过RA或无血清培养法诱导拟胚体分化为神经细胞。