The vector of narG gene targeting pEGude was introduced into denitrifier DNF409 by electrotransformation. An Erm~r Cm~s isolate DNF409EF was obtained and narG gene disruption was confirmed by PCR.

• 将基因中断载体pEGude电激转化至反硝化菌DNF409中，通过抗性筛选得到表型为Erm~r Cm~s的重组子DNF409EF，并通过PCR分析证实narG基因已被正确中断。