The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.

• 相关说明：PCRed TUB1-D252A products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210.