The virus was continually inoculated 5 generations onto rabbit kidney cell and chicken embryonic fibroblast cell (CEF) and induced typical cytopathic effect (CPE). The typical CPE cloud be seen when culturing the virus on RK-13 cell . PK-15cell .

• 该分离毒株在兔肾原代细胞上(RK)上和鸡胚成纤维细胞(CEF)上连传5代均出现典型细胞病变，再接种RK-13细胞、BHK-21细胞、Vero细胞、PK-15细胞和143 TK~-细胞均出现典型细胞病变，在RK-13细胞上的感染滴度为10~(6.；52)TCID_(50)/0