Then it was inserted into pBI-L vector to construct HGF and Smad7 expression vector containing luciferase reporter gene and respond element of tetracycline (TRE) by recombinant DNA technology.

• 利用DNA基因重组技术，将目的片段DNA插入含有荧光素酶报告基因和四环素反应元件的真核表达载体pBI-L中，分别构建真核表达载体pBI-L-HGF和pBI-L-Smad7，并进行酶切和测序鉴定。