Then the inclusion bodies were redissolved by 7 mol/L guanidine hydrochloride containing 10 mmol/L of DTT.

• 而稀释复性法是研究其它复性方法的基础，试验中先用含2 mol/L 尿素的0.；1 mol/L Tris-HCl 缓冲液对包涵体进行洗涤，然后用含10 mmol/L DTT 的7 mol/L盐酸胍溶解包涵体。