There was no different in culture between different timing of micromanipulation.Zygotes with good quality grew better.Zygotes with bad quality almost cannot grow.

• 结果：基因浓度越高，受精卵发育越差，操作时间不同的卵发育无明显差异，质量好的受精卵发育较好，质量差的几乎不发育。