Therefore, HepG2 - GS -3A, which overexpresses CYP 3A4 and GS, was applied to Kigunasu as our artificial liver to metabolize diazepam and ammonia.

• 人工生物肝脏循环器中培养HePGZ一GS一3A4细胞，并体外测定其安定与氨的代谢率。