Therefore, the present study was undertaken to explore the possibility of gene targeting in sheep fibroblasts by using myostatin as targeting gene, mAAT as the integrated gene and Neo as mark gene.

• 为此，本研究以myostatin基因为靶向基因，mAAT基因为目的基因，Neo基因为标记基因，对绵羊体细胞基因敲除进行了一系列探索性的研究。