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- Thermus aquaticus(Taq) 栖热水生菌, 嗜热水生菌
- Keywords Thermus aquaticus;Amylomaltase;gene cloning;prokaryotic expression; 水生栖热菌;麦芽糖转葡萄糖基酶;基因克隆;原核表达;
- Thermus aquaticus FL-03 水生栖热菌FL-03
- thermus aquaticus 粞热水生菌
- FastStart Taq DNA Polymerase, December ed. 酸外切酶活性( Roche Package Insert.
- Taq iqbux iqgx jauwinrx d’ gugqzuox zre'. (他一步一个脚印儿地工作着。
- The 16s r DNA sequence of YBJ-1 (151 Ibp in length) shares 98% identity with that of Thermus. 其16s rDNA(1511bp)序列与栖热菌(Thermus scotoductus ITI-252T)的同源性为98%25。
- It has been reported in foreign literature that the A 1 allele of the Taq I polymorphism of the dopamine D 2 receptor gene(DRD 2)was related to ADHD. 国外近年由于分子生物学方法的介入,发现多巴胺D2受体TaqIA1等位基因与本病相关。
- The site is famous for the remains of a gigantic vaulted hall, the Taq Kisra, which has one of the largest single-span brick arches in the world. 遗址有萨珊国王的宫殿,殿堂有世界最大的单跨砖拱之一。
- Method of DNA extraction and factors influencing RAPD analysis,including the concentration of DNA template, Mg 2+ , dNTP, primers and Taq, in jute were studied. 以假黄麻、假长果、越南圆果 (圆果种黄麻 )等为材料 ;研究了黄麻 DNA的提取方法以及对 RAPD分析的影响因素 ;包括模板浓度、Mg2 + 、d NTP、引物和 Taq酶等 ;建立了适于黄麻种质 RAPD分析的 PCR反应体系 .
- The frequencies distribution of Fok I, Aap I, Bsm I and Taq I alleles in 80 renal transplantation patients in Shanghai all in accordance with the Hardy-Weinberg equilibrium. 80例上海移植肾受者VDR基因四个多态位点中仅ApaI酶切位点的不同基因型间腰椎和股骨粗隆部位BMD有明显差异(p<0.;05);
- Methods Candida albicans was geno- typed using RAPD under the different conditions of primer,template,concentration of Mg~(2+)and dosage of Taq polymerase. 方法:选用不同的引物、Mg~(2+)、模板浓度、Taq聚合酶用量等,获得一优化的反应体系以对白假丝酵母菌进行RAPD分型。
- Methods HIV DNA and HIV RNA were detected by using Taq PCR,RT PCR,LD PCR,hybridization, and oligonucleotide 32 P probes against gag, tat, nef, LTRU 3 etc. 方法 应用PCR ,逆转录PCR ,长片段PCR ,分子杂交等技术 ,选用不同引物和32 P标记的gag、tat、nef、LTRU3等片段探针对HIVDNA存在状态、HIVRNA含量进行研究。
- The program also came under a taq(attack) after education department of(delete) officials were accused of conlax aventrist(conflicts of interest) with reading publishers. 这个项目在教育部官员被指控和读物出版商的利益冲突而收到攻击。
- Factors which affect the ISSR analysis of Abies beshanzuensis,such as annealing temperature and concentrations of template DNA,Taq DNA polymerase,Mg~2+ and dNTP,etc. were examined. 以百山祖冷杉DNA为材料;分析了DNA浓度、Mg2+浓度、dNTP浓度、Taq酶的含量以及退火温度对ISSRPCR扩增结果的影响;经过优化实验建立了百山祖冷杉ISSRPCR最佳反应体系.
- The ladder experiments showed: the best PCR amplified system of the crested ibis is as follows: Primer(10pmol/ul)lul, Taq enzyme(lU/ul)1.5ul, dNTP(2.5mM)2.0ul, Mg2+(25mM)2.5ul, teplate DNAlOng, TH2O17ul, and final volume is 25ul. 本试验对各种影响因素进行了梯度筛选,得到朱?最佳的PCR扩增体系为:引物(10pmol/ul)1ul、TaqDNA聚合酶(1U/ul)1.;5ul、dNTP(2
- Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays. The RTF PCR method was established to detect and identify GM maize lines. 根据转基因玉米T14/T25转入的外源基因质粒图谱,设计转基因引物和探针进行PCR和实时荧光PCR检测,建立了转基因玉米品系鉴定的实时荧光PCR方法。
- In Iraq, it operates in two fields in the semi-autonomous region of Kurdistan including the Taq Taq field, where it is sitting on 42.5 million barrels of proven and probable reserves. 在伊拉克,该公司在半自治的库尔德斯坦地区经营两座油田,包括已探明储量4250万桶的塔克塔克油田。
- Results:It was found that the concentrations and ratios of primers,the purity of DNA sample and the Taq polymerase employed seemed to be crucial for the accuracy of the HLA-A locus PCR-SSP. 结果:发现引物浓度和浓度比、DNA的纯度及酶的选用,是影响HLA-A位点PCR-SSP分型准确性的重要因素。
- Results :It was found that the concentrations and ratios of primers,the purity of DNA sample and the Taq polymerase employed seemed to be crucial for the accuracy of the HLA-A locus PCR-SSP. 结果 :发现引物浓度和浓度比、DNA的纯度及酶的选用,是影响HLA-A位点PCR-SSP分型准确性的重要因素。