They are S90,S171,S290,S359 and S1362.The genetic distances between the maize materials were further calculated with two methods, Nei & Li and Jaccard.

• 然后对20条引物进行筛选，结果得到6条具有重复性好、条带清晰、多态性高的引物，即S90、S171、S218、S290、S359、S1362。