Thirdly, 8 sera were detected by one-step ELISA again through shaking the microplate in 200 times/mm during incubation.

• 结果8例ELISA双抗原夹心一步法检测结果为阴性，怀疑存在钩状效应的患者血清标本，采用ELISA双抗原夹心二步法进行检测时，结果均转为阳性；