Thirdly, using RAPD technique to amplify DNA of the AALY001 NMS two lines, one specific marker NMSOPM09500 was obtained in the male fertile line but without in the male sterile line.

• 最后,选择万寿菊核不育两用系AALY001为材料,对雄性不育株和可育株进行扩增,得到了一条在不育株中缺失,在可育株中出现的稳定的特异标记NMSOPM09500。