Three detecting methods in yeast proteinase A (PrA) assay including UV absorbance, Lowry method and modified Bradford method were compared when using casein or hemoglobin as substrates.

• 其中以蛋白质为底物时的酶活性测定方法方便快捷,但是当酵母蛋白酶A酶活性较低时,产物采用紫外吸收法以及Lowry法的检测灵敏度都比较低。