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- CONSTRUCTION OF NOS/HuIFNa CHIMERIC GENE AND ITS FUSION WITH Ti PLASMID VECTOR NOS/HuIFNa嵌合基因的构建及其与Ti质粒载体的拼接
- Nopaline was inspected in the crown gall and this data showed that T-DNA of Ti plasmid had been transformed successfully . 栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长，并合成冠瘿碱，表明Ti质粒转化成功。
- Finally, the linear pBLI plasmid vector coated with liposomere agent,was transferred into the bFF to validate pBLI. 最后,以线性化的乳腺特异表达载体pBLI为外源基因,采用脂质体介导法转染牛胎儿成纤维细胞。
- The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. 科学家们利用基因抑制技术（knockdown）来降低oct4基因的表达，这是一种对老鼠和人类中胚胎干细胞进行自我更新非常关键的基因。
- By identifying the opines synthesized in the gall on tomato seedling incited by pathogenic strains, it was found that strains from peach, apple, hop beet and Chinese White poplar harboured nopaline type Ti plasmid. 对致病菌株的质粒类型的研究结果表明:桃树、啤酒花和甜菜菌株基本上都是胭脂碱(Nopaline)型质粒、葡萄菌株基本上是章鱼碱(Octopine)型质拉。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- We applied real-time image and fluorescence recovery after photobleaching (FRAP) to examine the nuclear pore permeability of 27 kDa EGFP in human lung cancer cells (ASTC-a-1) transfected with plasmid vector (pEGFP-C1). 本实验利用荧光漂白后恢复(FRAP,fluorescencerecoveryafterphotobleaching)法观测人肺腺癌肿瘤细胞(ASTC-a-1)中表达的27kDaEGFP在核质间的被动扩散,并以激光共焦显微镜进行实时成像。
- The recombinant Herpesviruses of Turkey transfer plasmid vector pT-C-gB-IL was cotransfected with Herpesviruses of Turkey at CEF by calcium phosphate-DNA coprecipitation method and the target fragment was inserted into TK domain of HVT. 确定转染时亲本毒的用量,用磷酸钙共沉淀法将HVT转移质粒载体pT-C-gB-IL与HVT在CEF细胞上进行同源重组,将目的基因导入HVT TK区。
- METHODS: Bcl-XL siRNA and negative siRNA plasmid vector were stably transfected into A549/DDP cells. Drug sensitivity of the cells to cisplatin(DDP) was analyzed with MTT and flow cytometry. 方法:将Bcl-XLsiRNA质粒载体转染至A549/DDP细胞,MTT、流式细胞仪检测细胞药物敏感性的改变;
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。
- tumor inducing plasmid(Ti plasmid) 根瘤诱导质粒, Ti质粒[根瘤农杆菌中能诱导植物产生冠瘿瘤的质粒]
- The Situation of Plasmid Vector in Gene Therapy 以质粒为载体的基因治疗现状
- Plasmid Vector Suitable for Gene Therapy or Immunization 一种基因治疗和基因免疫表达载体的构建
- CONSTRUCTION OF SOIL METEGENOMIC LIBRARY WITH PLASMID VECTOR 以质粒载体构建土壤宏基因组文库的研究
- Cloning and Sequencing of the VirD 1 Protein Gene Situating in Virus Region of Agribacterium Tumefaciens'Ti Plasmid 根癌土壤农杆菌Ti质粒毒性区VirD1基因的克隆及序列分析
- Human lung cancer cells (ASTC a 1) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800 mg/L G418. Strong fluorescence of five cell clones were observed after several generations. 以绿色荧光蛋白 (GFP)作为标记基因转入人类肺癌细胞系 (ASTC a 1) ;经 80 0mg/LG4 18筛选 ;获得5株高表达细胞系 .
- In this paper, the research in optimization design for DNA vaccine was reviewed, including choice of antigen gene and immune adjuvant, optimization of plasmid vectors and inoculation methods. 许多试图增强和调节质粒DNA疫苗的免疫原性和效率的方案都在研究之中。
- The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt 链霉菌和大肠杆菌穿梭质粒载体的构建
- ti plasmid ti 质粒
- Did't you know he has gone ti London? 难道你不知道提已经去伦敦了吗?
