To determine which transmembrane domain could locate PF40 in the ER, 4 truncated PF40 fragments were amplified by PCR and fused with gfp.

• 为了确定PF40的内质网定位区段,构建了pf40的4个不同长度的缺失片段(f12、f14、f56、f36)与报告基因gfp连接的植物表达载体,由农杆菌介导转化烟草。