Tonoplast-enriched vesicles were prepared fromsuspension-cultured Populus euphratica Oliv. cells by differential centrifugation and discontinuous sucrose density gradient centrifugation.

• 将悬浮培养的胡杨(Populus euphraticaOliv.；)细胞捣碎后，通过差速离心和不连续蔗糖密度梯度离心获得富集液泡膜的膜微囊。