Total RNA from extracted tooth germs was reverse-transcribed to generate cDNA probes and then hybridized with the mouse gene expression Microarray CSC-ME-80, and results were analyzed using the MIDAS software from chipsreen bioscienses ltd.

• 从小鼠第一磨牙牙胚提取总RNA；反转录得到cDNA探针；与小鼠基因表达谱芯片CSC-ME-80进行杂交；使用软件MIDAS分析芯片实验数据.