Total RNA of the tissues was extracted by TRIzol method (Gibco BRL). First strand cDNA was synthesized, using Superscript Preamplification System (Gibco BRL) according to the instruction.

• 按Trizol(GIBCO/BRL)试剂盒说明书操作提取组织总RNA，按Superscript Preamplification System for First Strand cDNA Synthesis(Gibco/BRL)试剂盒说明书要求的标准条件合成cDNA第一链。